Statoacoustic ganglion zebrafish. (B) Schematic drawing of the olfactory system in an adult zebrafish (dorsal view) and mouse (lateral view; sagittal section). Mar 15, 2010 · A previous report identified these cells as the statoacoustic ganglion (SAg) (Adamska et al. Epithelial projections forming the semicircular canal system protrude into the vesicle and fuse between 44 and 72h. Feb 19, 2023 · OXR1 encodes Oxidation Resistance 1 and is evolutionarily conserved from zebrafish to human. The statoacoustic ganglion (SAG) develops into several lobes that are spatially arranged to connect appropriatelywithhaircellsoftheinnerear. For instance, in adult zebrafish, organs like the heart, fin, retina, spinal cord, and several brain regions can regenerate without scarring [[2 - 6]]. Dec 15, 2006 · Statoacoustic ganglion cells in 36-38 hpf embryos were stained using antibody markers for neuronal differentiation that label the statoacoustic ganglia in zebrafish, anti-Hu antibodies (that recognize neuronal protein HuC/Hu; data not shown) and monoclonal antibody zn-5 (that recognizes neurolin/DMGRASP; Fig. We focused on neurovascular interactions in the statoacoustic ganglion (SAG), which comprises the sensory neurons that innervate mechanosensory hair cells of the inner ear and the brainstem. Reactivation of the Neurogenic Niche in the Adult Zebrafish Statoacoustic Ganglion Following a Mechanical Lesion Simone Schwarzer Devavrat Ravindra Rekhade Anja Machate Sandra Spieß Michaela Geffarth Diana Ezhkova Stefan Hans Frontiers in Cell and Developmental Biology doi 10. from publication: Hedgehog Signaling Governs the Development of Otic Sensory Epithelium and Its Associated Innervation in Zebrafish | The inner ear is Download scientific diagram | Acetylated tubulin staining of embryos at 3 dpf with control morpholino. Immunohistochemistry The following peripheral ganglia were examined: dorsal root ganglia (DRG), trigeminal and anterodorsal ganglia (V/AD g), facial and anteroventral ganglia (VII/AV g), statoacoustic ganglion In contrast, hair cells in the inner ear of zebrafish are produced throughout life and regenerate after trauma. Proper formation of the SAG is crucial for the establishment of appropriate connections with the sensory regions of the inner ear. Jun 28, 2023 · Morphological analyses of otic vesical, hair cells, statoacoustic ganglion and semicircular canals were conducted. To investigate the cellular behaviours involved in the 3D organization of the SAG, we use high-resolution confocal imaging of single-cell, labelled zebrafish neuroblasts (NBs), photoconversion Similar results have also been observed that the overexpression and knockdown of miR-194 strongly disturbed the development and differentiation of the sensory patches and Statoacoustic ganglion (SAG) through Fgf4 in zebrafish in vivo. Correction: Neurogenesis in the inner ear: the zebrafish statoacoustic ganglion provides new neurons from a Neurod/Nestin-positive progenitor pool well into adulthood (Development (Cambridge, England) (2020) 147 7 PII: dev191775) Apr 10, 2020 · In contrast, hair cells in the inner ear of zebrafish are produced throughout life and regenerate after trauma. Nov 8, 2023 · Highlighted Article: High-resolution confocal imaging and migration tracking of zebrafish neuroblasts reveals the cellular behaviours underlying statoacoustic morphogenesis and the role of pioneer neurons shaping the statoacoustic ganglion. Development of progenitors is regulated by Fgf, Wnt and … Dec 15, 2022 · Mutant embryos display behaviors associated with vestibular deficits and showed reduced number of anterior statoacoustic ganglion neurons that innervate the utricle, the vestibular organ in zebrafish. To investigate the cellular behaviours involved in the 3D organization of the SAG, we use high-resolution confocal imaging of single-cell, labelled zebrafish neuroblasts (NBs), photoconversion At this stage the medial lateral line ganglion and the vagal placode/ganglion also expressed cadherin-6 message, while the statoacoustic ganglion and the posterior lateral line ganglion continued expressing high levels of cadherin-6 message (Fig. Authors Baeza-Loya, S. Many factors . ag/tJl8268. Correction: Neurogenesis in the inner ear: the zebrafish statoacoustic ganglion provides new neurons from a Neurod/Nestin-positive progenitor pool well into adulthood (Development (Cambridge, England) (2020) 147 7 PII: dev191775) Jan 7, 2011 · statoacoustic ganglion (SAG) and fewer SAG neurons compared with control groups. In situ hybridization was further applied to verify the expression of genes of the related pathways. 2A) and often display 1-2 Mutant embryos display behaviors associated with vestibular deficits and showed reduced number of anterior statoacoustic ganglion neurons that innervate the utricle, the vestibular organ in zebrafish. Jul 31, 2023 · The lateral line system of zebrafish consists of the anterior lateral line, with neuromasts distributed on the head, and the posterior lateral line, with neuromasts distributed on the trunk. The observation that ingrowth of SAG neurites to presumptive sensory areas of the inner ear preceded cytodifferentiation of those receptor cells suggested a causal relationship. This week's Graduate Student Spotlight features Maria Ali, a student in Dr. Another neurotrophic cytokine, Monocyte chemoattractant protein 1 (MCP1) is known to synergize with MIF; but MCP1 alone is insufficient to support mouse/chick SAG neurite May 1, 2014 · (A) Schematic drawing of an adult zebrafish and mouse inner ear, their sensory patches and approximate position of the VIIIth ganglion (SAG). Toinvestigatethecellular behaviours involved in the 3D organization of the SAG, we use high-resolution confocal imaging of single-cell, labelled zebrafish neuroblasts (NBs), photoconversion, photoablation Cadherins are cell surface adhesion molecules that play important roles in development of tissues and organs. The primary afferent neurons in the statoacoustic cranial ganglion are the first stage of processing sound information encoded by the auditory hair cells and forward signals to the torus semicircularis located in the hindbrain (Higgs and Radford, 2013; Privat et al. the statoacoustic ganglion and vagal quences of cdh6 loss-of-function on cranial ganglion and nerve differentiation in zebrafish embryos. Zebrafish Anatomical Dictionary Structure description: statoacoustic ganglion by Tanya Whitfield Name: statoacoustic ganglion Abbreviation: sag, gVIII Synonyms: VIIIth nerve ganglion, octaval ganglion Figures: neurogenesis sections timelapse Description: The sensory ganglion of the ear. Mar 23, 2018 · Similar results have also been observed that the overexpression and knockdown of miR-194 strongly disturbed the development and differentiation of the sensory patches and Statoacoustic ganglion To test whether sox2 and sox3 play redundant or distinct roles in otic development, we examined accumulation of hair cells and neurons of the statoacoustic ganglion (SAG) in embryos disrupted for sox2 or sox3 or both sox2 and sox3. Apr 10, 2020 · Summary: This study provides the first comprehensive characterization of the zebrafish statoacoustic ganglion showing that, in sharp contrast to mammals, zebrafish display continued neurogenesis in the inner ear into adult stages. Embryos injected with zebrafish cdh6 specific antisense morpholino oligonucleotides (MOs, which suppress gene expression during development; cdh6 morphant embryos) displayed a specific phenotype, including (i) altered shape and reduced development of a subset of the cranial and lateral line ganglia (e. Apr 1, 2025 · During development of the vertebrate inner ear, sensory epithelia and neurons of the statoacoustic ganglion (SAG) arise from lineage-restricted progen… In contrast, hair cells in the inner ear of zebrafish are produced throughout life and regenerate after trauma. Statoacoustic ganglion neurones (SAG) are produced by the same group of cells (otic placode) that produce all of the receptor cells that populate the sensory areas of the inner ear. This dissertation focuses on the development and We discuss remaining questions in vestibular development and organization that are tractable in the zebrash model. OXR1 encodes Oxidation Resistance 1 and is evolutionarily conserved from zebrafish to human. Jan 1, 2020 · In contrast, hair cells in the inner ear of zebrafish are produced throughout life and regenerate after trauma. Mar 15, 2022 · Lesion of the Statoacoustic Ganglion via Injection Into the Otic Capsule To study the regenerative capacity of the zebrafish statoacoustic ganglion, we established a lesion assay based on fluid injection into the otic capsule. The neurogenic domain gives rise to the neurons of the VIIIth ganglion (statoacoustic ganglion, vestibuloacoustic ganglion, or vestibular and spiral ganglia, depending on species). Mar 1, 2022 · Recently, we showed that also inner ear neurogenesis continues to take place in the zebrafish statoacoustic ganglion (SAG) well into adulthood. Macrophage migration inhibitory factor (MIF) is a neurotrophic cytokine essential for inner ear hair cell (HC) development and statoacoustic ganglion (SAG) neurite outgrowth, and SAG survival in mouse, chick and zebrafish. Recently, we showed that also inner ear neurogenesis continues to take place in the zebrafish statoacoustic ganglion (SAG) well into adulthood. Neurogenesis in the inner ear: the zebrafish statoacoustic ganglion provides new neurons from a Neurod/Nestin-positive progenitor pool well into adulthood Article Mar 2020 Simone Schwarzer Nandini In this study we analyzed expression patterns of two δ-protocadherins, protocadherin-9 and protocadherin-17, in the developing zebrafish using in situ hybridization and RT-PCR methods. g. In our previous study of cadherin expression in the cranial ganglia and lateral line system of developing zebrafish, we found that the trigeminal (gV), anterodorsal ganglia (AD) and posterior lateral line ganglion (gP) began to express cadherin4 by 32 hpf, the statoacoustic ganglion (gVIII) became cadherin4-positive by 29 hpf (our most recent This antibody strongly labels cell bodies in the trigeminal ganglion (gV), anterodorsal lateral line ganglion (gAd), statoacoustic ganglion (sag) and posterior lateral line ganglion from 24 to 72 hpf zebrafish embryos (Raible and Kruse, 2000). , 2013). Apr 1, 2023 · The statoacoustic ganglion (SAG) displays several lobes spatially arranged to properly connect with hair cells of the inner ear. Highlighted Article: High-resolution confocal imaging and migration tracking of zebrafish neuroblasts reveals the cellular behaviours underlying statoacoustic morphogenesis and the role of pioneer neurons shaping the statoacoustic ganglion. Embryos injected with zebrafish cdh6 specific antisense morpholino oligonucleotides (MOs, which suppress gene expression during development; cdh6 morphant embryos) displayed a specific phenotype, including (i) altered shape and reduced May 16, 2011 · Embryos injected with zebrafish cdh6 specific antisense morpholino oligonucleotides (MOs, which suppress gene expression during development; cdh6 morphant embryos) displayed a specific phenotype, including (i) altered shape and reduced development of a subset of the cranial and lateral line ganglia (e. , 2015). The exact regulatory mechanism for the cell fate decisions in the zebrafish ear is uncertain. We therefore employed zebrafish models with known alterations in different NC populations to address whether gliogenic or chondrogenic NC affect ganglion assembly and neurogenesis. Western blot detection of calretinin in homogenates of whole adult Danio rerio. Previous studies support regulatory roles for Fgf and Wnt, but how signaling is coordinated is poorly understood. The vestibular system of the inner ear provides information about head motion and spatial orientation relative to gravity to ensure gaze stability, balance, and postural control. Keywords: anatomical map, cranial ganglia, head vasculature, statoacoustic ganglion, zebrafish Similar results have also been observed that the overexpression and knockdown of miR-194 strongly disturbed the development and differentiation of the sensory patches and Statoacoustic ganglion (SAG) through Fgf4 in zebrafish in vivo. (A – F) Whole mount in situ hybridization of embryos with an nkx5. Nov 8, 2023 · The statoacoustic ganglion (SAG) develops into several lobes that are spatially arranged to connect appropriately with hair cells of the inner ear. However, it is unknown whether new sensory neurons are also formed in the adult zebrafish statoacoustic ganglion (SAG), the sensory ganglion connecting the inner ear to the brain. Mar 21, 2002 · In the zebrafish, this placode cavitates to form a hollow ball of epithelium, the otic vesicle, from which all structures of the membranous labyrinth and the neurons of the statoacoustic (VIIIth) ganglion arise. Nov 1, 2002 · Expression of ngn1 delineates zebrafish ganglionic placodes, including trigeminal, lateral line, and epibranchial placodes. The extent of innervation by the statoacoustic ganglion was affected, as embryos that were injected and electroporated show decreased branching of the neurites (Figure 5). Generate reference Registered Authors Riley, Bruce, Whitfield, Tanya T. The sensory afferent neurons are contained in the anterior and In this study we analyzed expression patterns of two δ-protocadherins, protocadherin-9 and protocadherin-17, in the developing zebrafish using in situ hybridization and RT-PCR methods. 2- to 1. sox2 −/− mutants show a 25% reduction in the number of hair cells at 38. Fig. The zebrafish inner ear can be easily studied due to its quences of cdh6 loss-of-function on cranial ganglion and nerve differentiation in zebrafish embryos. A number of previously characterized mutations affect the prevalence of either chondrogenic or glial subtypes derived from NC. We mined the zebrafish genome exhaustively for genes encoding TRs and determined their expression in the zebrafish embryo by sequencing to saturation and in situ hybridisation (Armant et al. Abstract In zebrafish, sensory epithelia and neuroblasts of the inner ear form simultaneously in abutting medial and lateral domains, respectively, in the floor of the otic vesicle. 4E and G). Although the importance of Fgf, Wnt, and Hh for inner ear development have been Neurosensory Development in the Zebrafish Inner Ear Vemaraju, Shruti(2012-02-14) OXR1 encodes Oxidation Resistance 1 and is evolutionarily conserved from zebrafish to human. , 1998b). Nov 15, 2023 · We present the ‘zebrafish single-cell atlas of perturbed embryos’, single-cell trancriptomic data of developing zebrafish embryos across various timepoints and with genetic perturbations. Mar 1, 2018 · mESC mouse embryonic stem cells MCP1 monocyte chemoattractant protein 1 (also called CCL2) MIF macrophage migration inhibitory factor mif and mif-like in the zebrafish MO (s) morpholino antisense-oligonucleotide (s) NT-3 neurotrophin-3 ODF otocyst-derived factor p53 tumor suppressor gene PNS peripheral nervous system SAG statoacoustic ganglion SG spiral ganglion SGN spiral ganglion neurons Jan 1, 2020 · VIII th Nerve Development Neurons of the statoacoustic ganglion (SAG) are derived from neuroblasts of the developing otocyst, with the axons forming the VIIIth cranial nerve. Keywords: anatomical map, cranial ganglia, head vasculature, statoacoustic ganglion, zebrafish In contrast, hair cells in the inner ear of zebrafish are produced throughout life and regenerate after trauma. Experiment Conditions Description:light ablation: anterior lateral line ganglion, light ablation: statoacoustic (VIII) ganglion, chemical treatment by environment: leflunomide The otic ectoderm is neurogenic for the first-order neurons of the eighth cranial ganglion, the statoacoustic ganglion. Mar 1, 2012 · Here we show that the zebrafish mif pathway is required for both sensory hair cell (HC) and sensory neuronal cell survival in the ear, for HC differentiation, semicircular canal formation, statoacoustic ganglion (SAG) development, and lateral line HC differentiation. In contrast, in zebrafish ear development, an increase in statoacoustic ganglion neurons is accompanied by an increase, not a decrease, in hair cells in the two sensory macula of the Notch-defective mib mutant (Haddon et al. Reactivation of the Neurogenic Niche in the Adult Zebrafish Statoacoustic Ganglion Following a Mechanical Lesion Article Full-text available Mar 2022 Neurogenesis in the inner ear: the zebrafish statoacoustic ganglion provides new neurons from a Neurod/Nestin-positive progenitor pool well into adulthood FIGURE 1 | Establishment of a lesion paradigm of the adult zebrafish statoacoustic ganglion (SAG). A 20-gauge needle was used to open the skull before inserting a Hamilton syringe vertically approximately 1. However, imaging this process is challenging and the cell behaviours underpinning SAG morphogenesis are unclear. It is shown that adult zebrafish can efficiently regenerate brain lesions and lack permanent glial scarring, and regeneration after traumatic lesion of the adultZebrafish brain occurs efficiently from radial glia-type stem/progenitor cells. Neuroblasts of the statoacoustic ganglion (SAG) initially form in the floor of the otic vesicle during a relatively brief developmental window. To investigate the cellular behaviours involved in the 3D organization of the SAG, we use high-resolution confocal imaging of single-cell, labelled zebrafish neuroblasts (NBs), photoconversion Mutant embryos display behaviors associated with vestibular deficits and showed reduced number of anterior statoacoustic ganglion neurons that innervate the utricle, the vestibular organ in zebrafish. , 2019). (A) Zebrafish Prdm14 is localized to the nucleus, similar to human PRDM14. Zebrafish, in contrast to many mammals, display exceptional regenerative abilities [[1]]. New neuroblasts soon delaminate and migrate towards the The statoacoustic ganglion (SAG) develops into several lobes that are spatially arranged to connect appropriatelywithhaircellsoftheinnerear. Here, we review the major findings from recent work in zebrafish. Mar 31, 2004 · We are currently studying the effects of cadherin-2 knockdown on the statoacoustic ganglion (gVIII) development, together with its effect on the hair cell and supporting cell development, and the general morphology and gene expression patterns of the inner ear in the developing zebrafish, which will be the subject of a future manuscript. fi KEYWORDS zebrafish, vestibular, inner ear, statoacoustic ganglion, vestibular In contrast, hair cells in the inner ear of zebrafish are produced throughout life and regenerate after trauma. In We show that from 24 h post-fertilization (hpf) onwards, the statoacoustic ganglion (SAG) develops in direct contact with two main blood vessels, the primordial hindbrain channel and the lateral dorsal aortae (LDA). , the statoacoustic ganglion and vagal Aug 6, 2024 · Introduction Regeneration is a widespread phenomenon in the animal kingdom, yet various organisms exhibit differing capacities for it. To investigate the cellular behaviours involved in the 3D organization of the SAG, we use high-resolution confocal imaging of single-cell, labelled zebrafish neuroblasts (NBs), photoconversion In contrast, hair cells in the inner ear of zebrafish are produced throughout life and regenerate after trauma. b, c, Global UMAP embedding with cranial ganglia (n = 29,782 cells) and Rohon–Beard neurons in black (b, inset). Neurons of this ganglion innervate hair cells of the sensory placodes of the inner ear, their central processes form the VIIIth (octaval) nerve. Neuroblasts eventually differentiate and extend … Neurogenesis in the inner ear: the zebrafish statoacoustic ganglion provides new neurons from a Neurod/Nestin-positive progenitor pool well into adulthood Simone Schwarzer, Nandini Asokan, Oliver Bludau, Veronika Kuscha, Jan Kaslin§, Stefan Hans* CRTD - Center for Regenerative Therapies Dresden, Cluster of Excellence, Center for Molecular and Similar results have also been observed that the overexpression and knockdown of miR-194 strongly disturbed the development and differentiation of the sensory patches and Statoacoustic ganglion (SAG) through Fgf4 in zebrafish in vivo. Toinvestigatethecellular behaviours involved in the 3D organization of the SAG, we use high-resolution confocal imaging of single-cell, labelled zebrafish neuroblasts (NBs), photoconversion, photoablation This antibody strongly labels cell bodies in the trigeminal ganglion (gV), anterodorsal lateral line ganglion (gAd), statoacoustic ganglion (sag) and posterior lateral line ganglion from 24 to 72 hpf zebrafish embryos (Raible and Kruse, 2000). Nov 8, 2023 · KEY WORDS: CRISPR, Statoacoustic ganglion, Zebrafish, Inner ear, Delamination, Migration, RhoGTP ases, Neuroblasts INTRODUCTION The inner ear is responsible for the senses of hearing and balance Morphological analyses of otic vesical, hair cells, statoacoustic ganglion and semicircular canals were conducted. Another neurotrophic cytokine, Monocyte chemoattractant protein 1 (MCP1) is k … OXR1 encodes Oxidation Resistance 1 and is evolutionarily conserved from zebrafish to human. Otic The otic placode becomes subdivided into an anterior neurogenic and posterior non-neurogenic domain. May 13, 2009 · In the adult CNS, neurogenesis takes place in special niches. Fish were anesthetized in Tricaine (Sigma) diluted in fresh shwater. Download scientific diagram | Primary antibodies used for immunohistochemistry on sections. However, it is unknownwhether new sensory neuronsare alsoformed in the adult zebrafish statoacoustic ganglion (SAG), the sensory ganglion connecting the inner ear to the Mar 15, 2022 · In contrast, zebrafish produce sensory hair cells throughout life and additionally possess the remarkable capacity to regenerate them upon lesion. In contrast to mammals, stem cell niches are abundant in zebrafish and also found in other parts of the brain than telencephalon. We investigated this problem using pharmacological and transgenic methods to alter Fgf or Wnt Frontiers Frontiers in Cell and Developmental Biology Year: 2022 Month: March Volume: 10 Reactivation of the Neurogenic Niche in the Adult Zebrafish Statoacoustic Ganglion Following a Mechanical Lesion (C, D) Expression of the statoacoustic ganglion marker irx1b is similarly affected at 24 hpf. Moreover, a shox2-reporter fish showed labeling of developing statoacoustic ganglion neurons in the anterior macula of the otic vesicle. TFdb, the Transcription factor database for zebrafish, was build as an interface to link all these datasets and make them easily accessible. In zebrafish, auditory signals excite saccular hair cells, which are innervated by the afferent neurons of the statoacoustic ganglion (or VIIIth nerve). , 2011), and development of the statoacoustic ganglion (Kantarci, Edlund, Groves Oct 12, 2006 · INTRODUCTION The vertebrate inner ear is a conserved organ system comprising a series of interconnected chambers, each of which primarily mediates either vestibular or auditory function. Nov 1, 2006 · These neuroblasts will coalesce adjacent to the developing inner ear and begin to form the statoacoustic ganglion (SAG). Hair cells synapse with neurons of the VIIIth cranial ganglion, the statoacoustic ganglion (SAG), and transmit sensory information to the hindbrain. 1 probe, showing the otic Immunohistochemistry The following peripheral ganglia were examined: dorsal root ganglia (DRG), trigeminal and anterodorsal ganglia (V/AD g), facial and anteroventral ganglia (VII/AV g), statoacoustic ganglion (VIII g), glossopharyngeal ganglion (IX), vagal ganglion (X), posterolateral line ganglion (PL g), and sympathetic ganglia. During development of the vertebrate inner ear, sensory epithelia and neurons of the statoacoustic ganglion (SAG) arise from lineage-restricted progenitors that proliferate extensively before differentiating into mature post-mitotic cell types. Several cellular and molecular mechanisms have been elucidated Neurogenesis in the inner ear: The zebrafish statoacoustic ganglion provides new neurons from a Neurod/Nestin-positive progenitor pool well into adulthood Jan 1, 2016 · While anteriorly, these placodes will ultimately give rise to the adenohypophysis, the olfactory epithelium, and the lens of the eye; more posteriorly the otic placode will generate the inner ear and the statoacoustic ganglion (SAG), and the trigeminal and epibranchial placodes will provide sensory neurons that innervate the skin as well as Mar 17, 2023 · Using antisense morpholino oligonucleotides, Li et al. (A) Embryo without any treatment showed strong acetylated tubulin staining in the posterior Jan 29, 1996 · Neurons of the statoacoustic ganglion are first visible by staining with HNK-1 antibody at about 24 hours. It is not understood how these niches are formed during development and how they are maintained. SAG cells exhibit a complex pattern of development, regulation of which remains poorly understood. , the statoacoustic ganglion and vagal Mutant embryos display behaviors associated with vestibular deficits and showed reduced number of anterior statoacoustic ganglion neurons that innervate the utricle, the vestibular organ in zebrafish. What is the broader significance of your research? My research focuses on the developmental process of neurons found in the statoacoustic ganglion (SAG) in zebrafish. SAG Mar 23, 2018 · Similar results have also been observed that the overexpression and knockdown of miR-194 strongly disturbed the development and differentiation of the sensory patches and Statoacoustic ganglion (SAG) through Fgf4 in zebrafish in vivo. The antibody used recognizes a unique protein band with an estimated molecular weight of about 30 kDa. The statoacoustic ganglion (SAG) develops into several lobes that are spatially arranged to connect appropriately with hair cells of the inner ear. Apr 27, 2020 · Thus, lactate raises basal levels of MAPK and Etv5b (a critical effector of the Fgf pathway), rendering cells more responsive to dynamic changes in Fgf signaling required by many developing tissues. Keywords zebrafish; otic placode; otic vesicle; statoacoustic ganglion; lateral line; hair cell; supporting cell; hearing; balance; auditory; vestibular; cell death; retinoic acid; BMP; Claudins; Dach; Delta-Notch; Eya1; FGF; Krml1 MeSH Terms Key words: anatomical map; cranial ganglia; head vasculature; statoacoustic ganglion; zebrafish. If the auditory cues are sufficiently loud enough to startle, the signal is transmitted to the giant escape fiber neuron known as the Mauthner cell, which directly activates motor neurons Mar 31, 2004 · We are currently studying the effects of cadherin-2 knockdown on the statoacoustic ganglion (gVIII) development, together with its effect on the hair cell and supporting cell development, and the general morphology and gene expression patterns of the inner ear in the developing zebrafish, which will be the subject of a future manuscript. from publication: Reactivation of the Neurogenic Niche in the Adult Zebrafish Statoacoustic Ganglion In contrast, zebrafish produce sensory hair cells throughout life and additionally possess the remarkable capacity to regenerate them upon lesion. Statoacoustic ganglion in the mif , iclp morphants and 4-IPP treated embryos. Our results showed that direct delivery of morpholinos into In contrast, zebrafish produce sensory hair cells throughout life and additionally possess the remarkable capacity to regenerate them upon lesion. Our spatiotemporal anatomical map of the cranial ganglia and the head vasculature indicates physical interactions between both systems and suggests a possible functional interaction during development. Nov 1, 2023 · The statoacoustic ganglion (SAG) develops into several lobes that are spatially arranged to connect appropriately with hair cells of the inner ear. Neurogenesis in the inner ear: the zebrafish statoacoustic ganglion provides new neurons from a Neurod/Nestin-positive progenitor pool well into adulthood Article Mar 2020 Simone Schwarzer Nandini Nov 30, 2001 · ABSTRACT Recent years have seen a re-naissance of investigation into the mechanisms of inner ear development. Mutant embryos display behaviors associated with vestibular deficits and showed reduced number of anterior statoacoustic ganglion neurons that innervate the utricle, the vestibular organ in zebrafish. Finally, the ganglia derived from the epibranchial placodes emerge close to the branchial arches (Andermann et al. 1. How hair cells … Similar results have also been observed that the overexpression and knockdown of miR-194 strongly disturbed the development and differentiation of the sensory patches and Statoacoustic ganglion (SAG) through Fgf4 in zebrafish in vivo. In addition, ngn1 is expressed in a subset of cells within the otic vesicle that will delaminate to form the octaval (statoacoustic) ganglion. Genetic analysis of ze-brafish has contributed significantly to this en-deavour, with several dramatic advances re-ported over the past year or two. May 16, 2011 · Embryos injected with zebrafish cdh6 specific antisense morpholino oligonucleotides (MOs, which suppress gene expression during development; cdh6 morphant embryos) displayed a specific phenotype, including (i) altered shape and reduced development of a subset of the cranial and lateral line ganglia (e. (B) Schematic drawing of In contrast, zebrafish produce sensory hair cells throughout life and additionally possess the remarkable capacity to regenerate them upon lesion. , 2000). To investigate the cellular behaviors involved in the 3D organization of the SAG, we use high resolution confocal imaging of single cell labeled zebrafish neuroblasts (NB), photoconversion, photoablation and genetic Mar 15, 2022 · In contrast, zebrafish produce sensory hair cells throughout life and additionally possess the remarkable capacity to regenerate them upon lesion. Apr 10, 2020 · In contrast, hair cells in the inner ear of zebrafish are produced throughout life and regenerate after trauma. Recently, we showed that also inner ear neurogenesis continues to take place in the zebrash statoacoustic ganglion fi (SAG) well into adulthood. Riley's lab! Maria's research focuses on the developmental process of neurons found in the statoacoustic ganglion in zebrafish, learn more about Maria and her projects here! tx. , the statoacoustic ganglion and vagal Lesion of the Statoacoustic Ganglion via Injection Into the Otic Capsule To study the regenerative capacity of the zebra fish statoacoustic ganglion, we established a lesion assay based on uid injection fl into the otic capsule. The swimming pattern of lmo4a KO and MO zebrafish was tracked. Neuroblasts delaminate from the epithelium of the ventral floor of the otic vesicle to form the statoacoustic (VIIIth) ganglion between 22 and 42h. Search ZIRC Antibodies forBrowse 38 ZIRC Antibodies Mutant embryos display behaviors associated with vestibular deficits and showed reduced number of anterior statoacoustic ganglion neurons that innervate the utricle, the vestibular organ in zebrafish. (E, F) Examination of hair cells (hc) in control and morphant SqET4 transgenic larvae shows no effect Compared with less differentiated olfactory filaments in olfactory epithelia, the development of hair cells and statoacoustic ganglion neurons were normal. Moreover, a shox2 -reporter fish showed labeling of developing statoacoustic ganglion neurons in the anterior macula of the otic vesicle. Moderate misexpression of sox2 during placodal stages led to development of otic vesicles with expanded sensory and reduced neurogenic domains. 5-mm deep into the skull along the dorsal–ventral axis Nov 15, 2023 · Colours represent ganglia types: Tg, trigeminal ganglion; aLL, anterior lateral line ganglion; pLL, posterior lateral line; Epi, epibranchial ganglion; Sa, statoacoustic ganglion. 3389/fcell. Nov 11, 2023 · Highlighted Article: High-resolution confocal imaging and migration tracking of zebrafish neuroblasts reveals the cellular behaviours underlying statoacoustic morphogenesis and the role of pioneer neurons shaping the statoacoustic ganglion. 2022. Apr 1, 2019 · The statoacoustic ganglion is the sensory ganglion of the ear. Changes in the morphology of the statoacoustic ganglion were observed through acetylated tubulin staining. Neurons of the statoacoustic ganglion Morphological analyses of otic vesical, hair cells, statoacoustic ganglion and semicircular canals were conducted. Mar 10, 2010 · The inner ear is responsible for the perception of motion and sound in vertebrates. Keywords: CRISPR/Cas9; ENU mutagenesis; MAPK/Erk; developmental biology; etv5b; sensory epithelium; statoacoustic ganglion; zebrafish. Dec 12, 2022 · Mutant embryos display behaviors associated with vestibular deficits and showed reduced number of anterior statoacoustic ganglion neurons that innervate the utricle, the vestibular organ in zebrafish. To investigate the cellular behaviours involved in the 3D organization of the SAG, we use high-resolution confocal imaging of single-cell, labelled zebrafish neuroblasts (NBs), photoconversion Mar 17, 2015 · Author Summary Neurons of the statoacoustic ganglion (SAG) transmit impulses from the inner ear necessary for hearing and balance. INTRODUCTION Apr 18, 2023 · Keywords: zebrafish, vestibular, inner ear, statoacoustic ganglion, vestibular reflex Citation: Baeza-Loya S and Raible DW (2023) Vestibular physiology and function in zebrafish. We cloned zebrafish six1 and studied its function in inner ear Jul 31, 2023 · The primary afferent neurons in the statoacoustic cranial ganglion are the first stage of processing sound information encoded by the auditory hair cells and forward signals to the torus semicircularis located in the hindbrain (Higgs and Radford, 2013; Privat et al. In contrast, zebrafish produce sensory hair cells throughout life and additionally possess the remarkable capacity to regenerate them upon lesion. The general features of this process appear to be conserved in zebrafish, shown schematically in Figure 1A. Dec 13, 2017 · Our spatiotemporal anatomical map of the cranial ganglia and the head vasculature indicates physical interactions between both systems and suggests a possible functional interaction during development. Mar 15, 2022 · In contrast, zebrafish produce sensory hair cells throughout life and additionally possess the remarkable capacity to regenerate them upon lesion. Mar 12, 2020 · Request PDF | Neurogenesis in the inner ear: the zebrafish statoacoustic ganglion provides new neurons from a Neurod/Nestin-positive progenitor pool well into adulthood | The vertebrate inner ear Correction: Neurogenesis in the inner ear: the zebrafish statoacoustic ganglion provides new neurons from a Neurod/Nestin-positive progenitor pool well into adulthood (Development (Cambridge, England) (2020) 147 7 PII: dev191775). The kinocilia and stereocilia of hair cells, the innervation of hair cells, and the formation of ribbon synapses were also unaffected. Hair cells synapse with neurons of the statoacoustic ganglion (SAG), or the VIIIth cranial nerve, axons of which Nov 8, 2023 · The statoacoustic ganglion (SAG) is derived from neuroblasts that delaminate from the otic placode during development. Fish were anesthetized in Tricaine (Sigma) diluted in fresh fishwater. Both protocadherins were mainly detected in the embryonic Here we show that the zebrafish mif pathway is required for both sensory hair cell (HC) and sensory neuronal cell survival in the ear, for HC differentiation, semicircular canal formation, statoacoustic ganglion (SAG) development, and lateral line HC differentiation. May 1, 2014 · Otic The otic placode becomes subdivided into an anterior neurogenic and posterior non-neurogenic domain. Results from in vivo, in ovo and in Abstract The zebrafish otic vesicle initially forms with only two sensory epithelia, the utricular and saccular maculae, which primarily mediate vestibular and auditory function, respectively. Our mutant analysis in zebrafish showed that sox2 is uniquely required for sensory development while sox3 is uniquely required for neurogenesis. Oct 10, 2024 · Hair cells in the zebrafish inner ear are innervated by neurons in the statoacoustic ganglion (SAG), whereas hair cells in the lateral-line system are innervated by neurons in the anterior or posterior lateral-line ganglia (aLLg and pLLg) (Zecca et al. The statoacoustic ganglion is derived from cells that delaminate from the ventral floor of otic vesicle starting at 18 hpf (Haddon and Lewis, 1996). 5 hpf (Fig. In zebrafish, regularly patterned pigment stripes and an array of calcified scales form simultaneously in the skin during post-embryonic development. Knockdown and knockout of lmo4a both induced smaller otocysts, less hair cells, immature statoacoustic ganglion and malformed semicircular canals. Each chamber contains a sensory patch consisting of sensory hair cells and supporting cells. Possible candidates include members of the Pax–Six–Eya–Dach ( paired box–sine oculis homeobox–eyes absent–dachshund ) gene regulatory network. , Raible, D. 850624 3,320 views 2 citations Nov 1, 2024 · Declaration of sex- and gender-based analyses Sex determination in zebrafish occurs after juvenile stages, and no sex-determining genes have been described in zebrafish lines kept in the laboratory. Here, we test the role of pax5, which is preferentially expressed in the utricular macula. (2023) generated zebrafish with knockdown of oxr1b and analyzed morphant embryos for neurod1 (601724) and neurog1 (601726), markers for the statoacoustic ganglion (SAG) and posterior lateral line ganglion (pLL). Its functional unit, the sensory patch, contains mechanosensory hair cells innervated by sensory neurons from the statoacoustic ganglion (SAG) that project to the corresponding nuclei in the brainstem. These neurons are essential for hearing and vestibular function. (A) Scheme of the zebrafish head with the brain inside showing the injection position in relation to the brain and the SAG. Here we show that the zebrafish mif pathway is required for both sensory hair cell (HC) and sensory neuronal cell survival in the ear, for HC differentiation, semicircular canal formation, statoacoustic ganglion (SAG) development, and lateral line HC differentiation. To investigate the cellular behaviours involved in the 3D organization of the SAG, we use high-resolution confocal imaging of single-cell, labelled zebrafish neuroblasts (NBs), photoconversion Alsina, Berta Keywords anatomical map, cranial ganglia, head vasculature, statoacoustic ganglion, zebrafish MeSH Terms Animals Blood Vessels/embryology* Brain/blood supply* Brain/embryology* Cranial Nerves/blood supply* Cranial Nerves/embryology Ganglia/blood supply Ganglia/embryology Zebrafish/embryology* (all 9) PubMed 29235648 Full text @ J Neurogenesis in the inner ear: the zebrafish statoacoustic ganglion provides new neurons from a neurod/nestin-positive progenitor pool well into adulthood. Oct 28, 2005 · At this stage, the medial lateral line ganglion and the vagal placode/ganglion also expressed cadherin-6 message, while the statoacoustic ganglion and the posterior lateral line ganglion continued expressing high levels of cadherin-6 message (Fig. May 4, 2020 · Correction: Neurogenesis in the inner ear: the zebrafish statoacoustic ganglion provides new neurons from a Neurod/Nestin-positive progenitor pool well into adulthood Simone Schwarzer, Nandini Asokan, Oliver Bludau, Apr 18, 2023 · The vestibular system of the inner ear provides information about head motion and spatial orientation relative to gravity to ensure gaze stability, balance, and postural control. To investigate the cellular behaviours involved in the 3D organization of the SAG, we use high-resolution confocal imaging of single-cell, labelled zebrafish neuroblasts (NBs), photoconversion Neurons of the statoacoustic ganglion (SAG) transmit auditory and vestibular information from the inner ear to the hindbrain. From 22 hpf, these neuroblasts delaminate from the ventral otocyst and migrate to form a rudimentary ganglion (Haddon & Lewis, 1996). Possible candidates include members of the Pax–Six–Eya–Dach In contrast, zebrafish produce sensory hair cells throughout life and additionally possess the remarkable capacity to regenerate them upon lesion. The main components of the larval zebrafish ear are illustrated in Figure 1. Jan 7, 2011 · Changes in the morphology of the statoacoustic ganglion were observed through acetylated tubulin staining. In the embryonic chick, Prox1 is expressed in the statoacoustic ganglion (Stone et al, 2003), as it is in mouse. Jan 1, 2016 · This has been used to study multiple steps of zebrafish otic development, including specification of pre-placodal ectoderm (Kwon, Bhat, Sweet, Cornell, & Riley, 2010), otic placode induction (Padanad, Bhat, Guo, & Riley, 2012), sensory competence (Sweet et al. Both protocadherins were mainly detected in the embryonic In contrast, zebrafish produce sensory hair cells throughout life and additionally possess the remarkable capacity to regenerate them upon lesion. Keywords CRISPR/Cas9, Hair cells, Statoacoustic ganglion, atoh1a, neurog1 MeSH Terms Animals Fibroblast Growth Factors/metabolism Zebrafish*/genetics Zebrafish Proteins/genetics Wnt Signaling Pathway Gene Expression Regulation, Developmental Ear, Inner*/metabolism PAX2 Transcription Factor/genetics PAX2 Transcription Factor/metabolism (all 9 Prdm14 is a nuclear protein and prdm14 is expressed before islet2 in zebrafish embryos. They soon delaminate and undergo a protracted phase Embryos injected with zebrafish cdh6 specific antisense morpholino oligonucleotides (MOs, which suppress gene expression during development; cdh6 morphant embryos) displayed a specific phenotype, including (i) altered shape and reduced development of a subset of the cranial and lateral line ganglia (e. Magenta dotted line and asterisk in ouabain-injected SAG indicates a massive (Continued ) - "Reactivation of the Neurogenic Niche in the Adult Zebrafish Statoacoustic Ganglion Following a Mechanical Lesion" A comparison of Prox1 expression in the developing mouse inner ear with that of the chick and the fish highlights some interesting similarities across vertebrate development. Development, 147(7), April 2020. Nov 1, 2012 · Neuroblasts of the statoacoustic ganglion (SAG) initially form in the floor of the otic vesicle during a relatively brief developmental window. Dec 21, 2022 · Mutant embryos display behaviors associated with vestibular deficits and showed reduced number of anterior statoacoustic ganglion neurons that innervate the utricle, the vestibular organ in zebrafish. The inner ear houses sensory epithelia composed of mechanosensory hair cells and non-sensory support cells. ALLg: Anterior lateral line ganglion. The ganglion neuroblasts are the earliest recognizable cell type; they delaminate from the otic ectoderm at the otic cup stage even before it completes vesicle formation. During early inner ear development, patterning of the sensory and neural domains is established in response to multiple signaling inputs. ID ZDB-PUB-230505-49 Date 2023 Source Frontiers in cell and developmental biology 11: 11729331172933 (Review) Generate reference Registered Authors Keywords inner ear, statoacoustic ganglion, vestibular, vestibular reflex, zebrafish MeSH Terms none PubMed 37143895 Full text @ Front Cell Dev Biol Citation Recently, we showed that also inner ear neurogenesis continues to take place in the zebrafish statoacoustic ganglion (SAG) well into adulthood. The slides were stained with anti-GFP (green) and DAPI (blue). 4 E,G). Morpholino knockdown of pax5 disrupts vestibular function but not hearing. Nov 15, 2012 · Development of the statoacoustic ganglion (SAG) The paradigm for otic neurogenesis, as formalized in several recent reviews [17], [18], involves a sequential process of specification, delamination, proliferative expansion and differentiation of precursor cells to form the mature SAG. Here we show that transcription factor Tfap2a coordinates multiple cell signaling pathways needed to regulate the quantity and pace of SAG neuron production. SAG neuroblasts originate in the floor of the otic vesicle. The focus of this chapter will be on recent advances in our understanding of otic development and function in the zebrafish, together with findings that have come from its use as a disease model and for drug In contrast, zebrafish produce sensory hair cells throughout life and additionally possess the remarkable capacity to regenerate them upon lesion. Here, Aitor Baňón and Berta Similar results have also been observed that the overexpression and knockdown of miR-194 strongly disturbed the development and differentiation of the sensory patches and Statoacoustic ganglion (SAG) through Fgf4 in zebrafish in vivo. Introduction The central nervous system (CNS) is vascularized through angiogenic vessels growing from a perineural vascular plexus. Jan 1, 2014 · The zebrafish is now a well-established model for the study of the development, pathology, and regeneration of almost all vertebrate organ systems, and the inner ear is no exception. I do not anticipate sex-biases to affect the main conclusions of this work. The vertebrate inner ear is a complex structure responsible for hearing and balance. Sep 20, 2022 · OXR1 encodes Oxidation Resistance 1 and is evolutionarily conserved from zebrafish to human. Oct 11, 2006 · The developmental processes leading to the differentiation of mechanosensory hair cells and statoacoustic ganglion neurons from the early otic epithelium remain unclear. The trigeminal ganglion (Tg) is the first one to appear, followed by the statoacoustic ganglion (SAG) and lateral line ganglia (LL). We found that the ortholog oxr1b gene is expressed in the statoacoustic ganglion (SAG, a sensory ganglion of ear) and posterior lateral line ganglion (pLL) in zebrafish. Mar 1, 2022 · J-K: Immunostaining of EGFP on neuromasts, statoacoustic ganglion, and pituitary in frozen sections of ET189b larvae at 5 dpf. W. 2002). In mammals, damaged hair cells and neurons are not regenerated. Zebrafish, like humans, have five sensory patches per ear that serve as peripheral vestibular organs, with the addition of the lagena and macula neglecta. Embryos injected with zebrafish cdh6 specific antisense morpholino oligonucleotides (MOs, which suppress gene expression during development; cdh6 morphant embryos) displayed a specific phenotype, including (i) altered shape and reduced Results lmo4a is constitutively expressed in the otic placode and otic vesicle during the early stages of zebrafish development. Recently, we showed that also inner ear neurogenesis continues to take place in the zebrafish statoacoustic ganglion (SAG) well into adulthood. 4). Download scientific diagram | (A) Schematic drawing of an adult zebrafish and mouse inner ear, their sensory patches and approximate position of the VIIIth ganglion (SAG). Zebrafish, like humans, have five sensory patches per ear that serve as peripheral vestibular organs, with the addition o … May 4, 2020 · Correction: Neurogenesis in the inner ear: the zebrafish statoacoustic ganglion provides new neurons from a Neurod/Nestin-positive progenitor pool well into adulthood Mutant embryos display behaviors associated with vestibular deficits and showed reduced number of anterior statoacoustic ganglion neurons that innervate the utricle, the vestibular organ in zebrafish. To understand common characteristics of neural stem cell niches in vertebrates, we studied the origin and Mar 3, 2023 · OXR1 encodes Oxidation Resistance 1 and is evolutionarily conserved from zebrafish to human. Mar 1, 2018 · Macrophage migration inhibitory factor (MIF) is a neurotrophic cytokine essential for inner ear hair cell (HC) development and statoacoustic ganglion (SAG) neurite outgrowth, and SAG survival in mouse, chick and zebrafish. Serial sections and time-lapse films show that the neuronal precursors originate by delamination from the ventral face of the otocyst; the peak period of delamination is from 22 hours to 30 hours. In contrast, hair cells in the inner ear of zebrafish are produced throughout life and regenerate after trauma. In this study we analyzed expression pattern of cadherin10, a member of the type II classic cadherin subfamily, in the embryonic zebrafish using Jun 28, 2023 · Morphological analyses of otic vesical, hair cells, statoacoustic ganglion and semicircular canals were conducted. They soon delaminate and undergo a protracted phase of proliferation and migration (transit-amplification). 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